ASO (Anti Streptolysin O) Test

 ASO (Anti Streptolysin O) Test

Streptolysin O is a toxic immunogenic exoenzyme produced by beta hemolytic streptococcus group A, C and G.  ASO or Anti Streptolysin O antibody is useful for the diagnosis of Rheumatic fever, acute glomerulonephritis and streptococcal infection.  Rheumatic fever is an inflammatory disease affecting connective tissue in several parts of human body such as skin, heart, joints, etc.  Acute glomerulonephritis is a renal condition that affect mainly renal glomerulus.

Aim

Determination of ASO or Anti Streptolysin O antibody in serum.

Materials required

·         ASO Antigen

·         ASO Positive control – human pooled serum – 1 x 0.5 ml – sample A

·         ASO negative control – human pooled serum – 1 x 0.5 ml – sample B

·         Glycine Saline Buffer Concentrate – 1 x 5 ml

·         Reaction slides

·         Applicator sticks

·         Serum Droppers

·         Rubber teat

Procedure

1.      All the reagents and the samples were allowed to reach room temperature and mixed well

2.      One drop of serum sample was placed on the slide with the help of a disposable serum dropper (Sample A and B)

3.      One drop of ASO Latex antigen was added to the above drops and mixed using an applicator stick

4.      The slide was rocked gently to and fro for 2 minutes and it was examined for agglutination under light

Result

The appearance of agglutination indicates the presence of specific antibody (ASO) in the serum sample.